Difference between revisions of "Part:BBa K1075012:Design"

 
Line 12: Line 12:
 
===Source===
 
===Source===
  
It was amplified from a plasmid from Addgene - provided by Klaus Hahn. LOV2 originally comes from ''Avena sativa'' while the ipaA peptide comes from Shigella spp.
+
It was amplified from a plasmid from Addgene - provided by Klaus Hahn. LOV2 originally comes from ''Avena sativa'' while the ipaA peptide comes from ''Shigella spp''.
  
 
===References===
 
===References===
 
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334866/]Lungu et al. (2012) Designing photoswitchable peptides using the AsLOV2 domain
 
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334866/]Lungu et al. (2012) Designing photoswitchable peptides using the AsLOV2 domain

Latest revision as of 10:21, 4 October 2013

AsLOV2-ipaA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the fusion protein was designed by Lungu et al. in 2012


Source

It was amplified from a plasmid from Addgene - provided by Klaus Hahn. LOV2 originally comes from Avena sativa while the ipaA peptide comes from Shigella spp.

References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334866/]Lungu et al. (2012) Designing photoswitchable peptides using the AsLOV2 domain