Difference between revisions of "Part:BBa K1075012"

Revision as of 10:19, 4 October 2013

AsLOV2-ipaA

Fusion of LOV 2 (A. sativa) with the ipaA peptide designed by Lungu et al. in 2012. It forms light inducable a tight dimer with Vinculin.

In had been shown that the LOV domain can be fused to entire protein domains, allowing photomodulation of the protein binding. However, Lungu et al. (2012) stated that it might be of high importance to bring the LOV domain closer to ipaA, in order to allow photomodulation in this case, because ipaA is only a peptide and thus more flexible than folded domains.

Therefore, Lungu et al. (2012) identified similar amino acid sequences in the ipaA peptide and the Ja helix of the LOV Domain and used this combined with molecular modeling to create photomodulateable AsLOV2-ipaA.

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334866/]Lungu et al. (2012) Designing photoswitchable peptides using the AsLOV2 domain

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1075012 short</partinfo>
-Fusion of LOV 2 (A. sativa) with the ipaA peptide. It forms light inducable a tight dimer with Vinculin. [1]
+Fusion of LOV 2 (''A. sativa'') with the ipaA peptide designed by Lungu et al. in 2012. It forms light inducable a tight dimer with Vinculin.
+In had been shown that the LOV domain can be fused to entire protein domains, allowing photomodulation of the protein binding. However, Lungu et al. (2012) stated that it might be of high importance to bring the LOV domain closer to ipaA, in order to allow photomodulation in this case, because ipaA is only a peptide and thus more flexible than folded domains.
+Therefore, Lungu et al. (2012) identified similar amino acid sequences in the ipaA peptide and the Ja helix of the LOV Domain and used this combined with molecular modeling to create photomodulateable AsLOV2-ipaA.
+[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334866/]Lungu et al. (2012) Designing photoswitchable peptides using the AsLOV2 domain
 <!-- Add more about the biology of this part here