Difference between revisions of "Part:BBa K1075011:Design"
 
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===Design Notes===
 
===Design Notes===
 
We used the same amino acid sequence as used for the light inducible heterodimerisation system from Lungu et al. (2012).
 
We used the same amino acid sequence as used for the light inducible heterodimerisation system from Lungu et al. (2012).
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−
 
  
 
===Source===
 
===Source===

Latest revision as of 09:59, 4 October 2013

Vinculin D1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the same amino acid sequence as used for the light inducible heterodimerisation system from Lungu et al. (2012).

Source

It was PCR amplified from a plasmid containing full length Vinculin from M. musculus.

References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334866/]Lungu et al. (2012) Designing photoswitchable peptides using the AsLOV2 domain

[http://www.cell.com/retrieve/pii/0092867479903684]Geiger B. (1979) A 130K protein from chicken gizzard: its localization at the termini of microfilament bundles in cultured chicken cells.