Difference between revisions of "Part:BBa K1075011:Design"

 
 
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<partinfo>BBa_K1075011 short</partinfo>
 
<partinfo>BBa_K1075011 short</partinfo>
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===Design Notes===
 
===Design Notes===
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We used the same amino acid sequence as used for the light inducible heterodimerisation system from Lungu et al. [1]
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We used the same amino acid sequence as used for the light inducible heterodimerisation system from Lungu et al. (2012).
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===Source===
 
===Source===
  
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It was PCR amplified from a plasmid containing full length Vinculin from M. musculus.
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It was PCR amplified from a plasmid containing full length Vinculin from ''M. musculus''.
  
 
===References===
 
===References===
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[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334866/]Lungu et al. (2012) Designing photoswitchable peptides using the AsLOV2 domain
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[http://www.cell.com/retrieve/pii/0092867479903684]Geiger B. (1979) A 130K protein from chicken gizzard: its localization at the termini of microfilament bundles in cultured chicken cells.

Latest revision as of 09:59, 4 October 2013

Vinculin D1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the same amino acid sequence as used for the light inducible heterodimerisation system from Lungu et al. (2012).

Source

It was PCR amplified from a plasmid containing full length Vinculin from M. musculus.

References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334866/]Lungu et al. (2012) Designing photoswitchable peptides using the AsLOV2 domain

[http://www.cell.com/retrieve/pii/0092867479903684]Geiger B. (1979) A 130K protein from chicken gizzard: its localization at the termini of microfilament bundles in cultured chicken cells.