Difference between revisions of "Part:BBa K1075046:Design"
(→Design Notes) |
|||
| Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | This part is a combination of the light-inducible <partinfo>BBa_K1075044</partinfo> and the toxin mazF (Muñoz-Gómez et al 2004). | + | This part is a combination of the light-inducible <partinfo>BBa_K1075044</partinfo> and the toxin mazF (<partinfo>BBa_K1075037</partinfo>, Muñoz-Gómez et al 2004). |
azF is a toxin, which is counteracted by its antitoxin MazE. MazF exhibits sequence-specific ribonuclease activity toward single- or double-stranded RNA regions, and the resulting degradation of cellular mRNA causes global translation inhibition. (Zhang et al. 2003) | azF is a toxin, which is counteracted by its antitoxin MazE. MazF exhibits sequence-specific ribonuclease activity toward single- or double-stranded RNA regions, and the resulting degradation of cellular mRNA causes global translation inhibition. (Zhang et al. 2003) | ||
Latest revision as of 09:47, 4 October 2013
pDawn-RBS32-mazF-TT
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2171
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 63
Illegal NgoMIV site found at 195
Illegal NgoMIV site found at 289
Illegal NgoMIV site found at 582
Illegal NgoMIV site found at 1076
Illegal NgoMIV site found at 1094
Illegal NgoMIV site found at 1184
Illegal AgeI site found at 414
Illegal AgeI site found at 1542 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1643
Illegal BsaI.rc site found at 525
Design Notes
This part is a combination of the light-inducible BBa_K1075044 and the toxin mazF (BBa_K1075037, Muñoz-Gómez et al 2004).
azF is a toxin, which is counteracted by its antitoxin MazE. MazF exhibits sequence-specific ribonuclease activity toward single- or double-stranded RNA regions, and the resulting degradation of cellular mRNA causes global translation inhibition. (Zhang et al. 2003)
Source
The origin of the mazEF (chpA) toxin-antitoxin system is the bacteria Escherichia coli. Here it is involved in the cell response to nutritional and antibiotic stresses as well as in bacterial-programmed cell death.
References
[http://www.sciencedirect.com/science/article/pii/S0014579304005885]Muñoz-Gómez AJ, Santos-Sierra S, Berzal-Herranz A, Lemonnier M, Díaz-Orejas R.(2004)Insights into the specificity of RNA cleavage by the Escherichia coli MazF toxin.
[http://www.cell.com/molecular-cell/retrieve/pii/S1097276503004027]Zhang Y, Zhang J, Hoeflich KP, Ikura M, Qing G, Inouye M.(2003)MazF Cleaves Cellular mRNAs Specifically at ACA to Block Protein Synthesis in Escherichia coli.