Difference between revisions of "Part:BBa K1075044"
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<partinfo>pDawn</partinfo>
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<partinfo>BBa_K1075044</partinfo>
  
 
pDawn can be used to induce transcription of a downstream gene with blue light.
 
pDawn can be used to induce transcription of a downstream gene with blue light.
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</br></br>
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The plasmid pDawn was designed by Ohlendorf et al.<sup><a href=#ref21.1>21.1</a></sup> in 2012 together with its counter plasmid pDusk. Both plasmids are single plasmid systems, which allow the activation (pDawn) or repression (pDusk) of gene expression by blue light. They are easy to implement in the laboratory and lead to up to 460-fold activity change upon ilumination.
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The plasmid pDawn was designed by Ohlendorf et al. in 2012 together with its counter plasmid pDusk. Both plasmids are single plasmid systems, which allow the activation (pDawn) or repression (pDusk) of gene expression by blue light. They are easy to implement in the laboratory and lead to up to 460-fold activity change upon ilumination.
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</br></br><h2>References</h2></br>
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<p><a name=ref21.1>21.1</a> <a href=http://www.sciencedirect.com/science/article/pii/S0022283612000113> Ohlendorf et al. (2009) From Dusk till Dawn One-Plasmid Systems for Light-Regulated Gene Expression.</a></p>
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'''fett'''References'''/fett'''
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Ohlendorf et al. (2009) From Dusk till Dawn One-Plasmid Systems for Light-Regulated Gene Expression.[http://www.sciencedirect.com/science/article/pii/S0022283612000113]
  
 
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<!-- Add more about the biology of this part here

Revision as of 08:22, 4 October 2013

BBa_K1075044

pDawn can be used to induce transcription of a downstream gene with blue light.

The plasmid pDawn was designed by Ohlendorf et al. in 2012 together with its counter plasmid pDusk. Both plasmids are single plasmid systems, which allow the activation (pDawn) or repression (pDusk) of gene expression by blue light. They are easy to implement in the laboratory and lead to up to 460-fold activity change upon ilumination.

fettReferences/fett

Ohlendorf et al. (2009) From Dusk till Dawn One-Plasmid Systems for Light-Regulated Gene Expression.[http://www.sciencedirect.com/science/article/pii/S0022283612000113]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
    Illegal NgoMIV site found at 195
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 582
    Illegal NgoMIV site found at 1076
    Illegal NgoMIV site found at 1094
    Illegal NgoMIV site found at 1184
    Illegal AgeI site found at 414
    Illegal AgeI site found at 1542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1643
    Illegal BsaI.rc site found at 525