Difference between revisions of "Part:BBa K1065100"
 
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<partinfo>BBa_K1065100 short</partinfo>
 
<partinfo>BBa_K1065100 short</partinfo>
  
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This BioBrick contains the coding sequence for BSMT1 (S-adenosylmethionine-dependent methyltransferase), it was derived from a Petunia hybrida gene and it catalyzes the reaction neeeded to product Methyl Salicylate from Salicylic Acid. This part was cloned by the iGEM Trento 2013 team for the creation of Wintergreen Device <partinfo>BBa_K1065106</partinfo>.  
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This BioBrick contains the coding sequence for BSMT1 (SAM benzoic acid/salicylic acid carboxyl methyltransferase I), it was derived from a Petunia x hybrida gene and it catalyzes the reaction neeeded to product Methyl Salicylate from Salicylic Acid. This part was cloned by the iGEM UniTN Trento 2013 team for the creation of Wintergreen Devices <partinfo>BBa_K1065106</partinfo> and <partinfo>BBa_K1065106</partinfo>.  
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<br>
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It was extrated from the iGEM Registry 2012 and
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This part was submitted in pSB1C3 to the registry by the UniTN-Trento 2013, using an original part of iGEM MIT 2006 <partinfo>BBa_J45119</partinfo> in pSB1AT3.
 
   
 
   
  
 
===Usage and Biology===
 
===Usage and Biology===
  
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<center><img style="width:800px;"src="https://static.igem.org/mediawiki/2013/c/c4/Tn-2013_ETH_detection.jpg"></center>
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BSMT1 catalyzes the conversion of salicylic acid to methyl salicylate, which has a wintergreen smell.
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<center><p style="width:600px; margin-bottom:60px; text-align:justify"><b>Figure 2</b>  
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<html>
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<center><img style="width:500px;"src="https://static.igem.org/mediawiki/parts/6/66/Tn-2013meth.jpg"></center>
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<center><p style="width:600px; margin-bottom:60px; text-align:justify">.
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</html>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K1065100 parameters</partinfo>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1065100 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1065100 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
 
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===Functional Parameters===
 
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<partinfo>BBa_K1065100 parameters</partinfo>
 
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Latest revision as of 18:32, 3 October 2013

BSMT1 enzyme

This BioBrick contains the coding sequence for BSMT1 (SAM benzoic acid/salicylic acid carboxyl methyltransferase I), it was derived from a Petunia x hybrida gene and it catalyzes the reaction neeeded to product Methyl Salicylate from Salicylic Acid. This part was cloned by the iGEM UniTN Trento 2013 team for the creation of Wintergreen Devices BBa_K1065106 and BBa_K1065106.
This part was submitted in pSB1C3 to the registry by the UniTN-Trento 2013, using an original part of iGEM MIT 2006 BBa_J45119 in pSB1AT3.


Usage and Biology

BSMT1 catalyzes the conversion of salicylic acid to methyl salicylate, which has a wintergreen smell.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 682
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 223