Difference between revisions of "Part:BBa K1123005:Experience"

Revision as of 17:26, 3 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1123005

As part of the iGEM 2013 competition the TU-Eindhoven team attempted to create a promoter which would induce protein expression under anaerobic conditions. This functional promoter could then be used to express CEST proteins (which should enable MRI imaging) upon the bacterium entering tumour regions. To test the functionality of the promoter we decided to express EGFP under anaerobic conditions. EGFP can be easily analysed making it easy for us to characterize the workings of the FNR promoter.

If we manage to express EGFP using this FNR promoter: BBa_K1123000

User Reviews

UNIQ21a26dcec60983ca-partinfo-00000000-QINU UNIQ21a26dcec60983ca-partinfo-00000001-QINU

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 ===Applications of BBa_K1123005===
-As part of the iGEM 2013 competition the TU-Eindhoven team attempted to create a promoter which would induce protein expression under anaerobic conditions. This functional promoter could then be used to express CEST proteins (which should enable MRI imaging) upon the bacterium entering tumour regions. To test the functionality of the promoter ex-vivo
+As part of the iGEM 2013 competition the TU-Eindhoven team attempted to create a promoter which would induce protein expression under anaerobic conditions. This functional promoter could then be used to express CEST proteins (which should enable MRI imaging) upon the bacterium entering tumour regions. To test the functionality of the promoter we decided to express EGFP under anaerobic conditions. EGFP can be easily analysed making it easy for us to characterize the workings of the FNR promoter.
+If we manage to express EGFP using this FNR promoter: [[Part:BBa_K1123000 | BBa_K1123000]]
 ===User Reviews===