Difference between revisions of "Part:BBa K1075020"

Revision as of 16:15, 3 October 2013

E. coli ssrA(DAS+4)

fused to the C terminus of a protein it triggers degradation upon induction of sspB function.

construction

The part contains the mutated ssrA(DAS+4) tag.

biology

The ssrA tag is a short peptide sequence, which is fused to the C-terminus of proteins, which should be degraded. It relates to the protease ClpXP complex in E.coli and it also allows the adaptor proteins SspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutated ssrA (DAS+4) weakens a direct binding between proteases and ssrA and increases the dependance of sspB. http://www.ncbi.nlm.nih.gov/pubmed/16762842

application

As we want to control protein degradation by controlling the function of SspB, we tag the red fluorescent protein mCherry with ssrA (DAS+4) to measure the degradation rate.

Sequence and Features