Difference between revisions of "Part:BBa K1104204:Experience"
| Line 3: | Line 3: | ||
This part: AhpCp2D1 is one of the improvement versions of BioBrick Part: ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCp2D1 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing ''Nosema ceranae'']. | This part: AhpCp2D1 is one of the improvement versions of BioBrick Part: ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCp2D1 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing ''Nosema ceranae'']. | ||
| − | [[File: NYMU_44.png|frame|center|''' | + | [[File: NYMU_44.png|frame|center|'''AhpCp2D1 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104244 Part:BBa_K1104244])''']] |
We use the device: AhpCp2D1 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104244 Part:BBa_K1104244]) includes promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induce the promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identify the strength. After AhpCp1000 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP. | We use the device: AhpCp2D1 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104244 Part:BBa_K1104244]) includes promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induce the promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identify the strength. After AhpCp1000 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP. | ||
Revision as of 11:47, 3 October 2013
This part: AhpCp2D1 is one of the improvement versions of BioBrick Part: ahpC promoter (Part:K362001) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCp2D1 (Part:BBa_K1104204) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].
We use the device: AhpCp2D1 +E0840 (Part:BBa_K1104244) includes promoter (Part:BBa_K1104204) and Part:BBa_E0840, induce the promoter by different concentrations of H2O2, and identify the strength. After AhpCp1000 (Part:BBa_K1104204) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.
We tested the improved promoter: AhpCp2D1 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing.
Results
Here is our result of AhpCp2D1 testing:
Discussion
Applications of BBa_K1104204
User Reviews
UNIQ11093ec7349bf3d2-partinfo-00000000-QINU UNIQ11093ec7349bf3d2-partinfo-00000001-QINU