Difference between revisions of "Part:BBa K1104205:Experience"
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| + | This part: AhpCp2 is one of the improvement versions of BioBrick Part: ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCp2 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104205 Part:BBa_K1104205]) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing ''Nosema ceranae'']. | ||
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| + | [[File: NYMU_45.png|frame|center|'''AhpCp2 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104245 Part:BBa_K1104245])''']] | ||
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| + | We use the device: AhpCp2 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104245 Part:BBa_K1104245]) includes promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104205 Part:BBa_K1104205]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induce the promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identify the strength. After AhpCp2 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104205 Part:BBa_K1104205]) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP. | ||
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| + | We tested the improved promoter: AhpCp2 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing. | ||
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==Results== | ==Results== | ||
Here is our result of AhpCp2 testing: | Here is our result of AhpCp2 testing: | ||
[[File: NYMU_AhpCp2_strength.png|frame|center]] | [[File: NYMU_AhpCp2_strength.png|frame|center]] | ||
| − | [[File: NYMU_all1.png|frame|center]] | + | [[File: NYMU_all1.png|frame|center|Comparison between ahpC and its improvements. ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104245 Part:BBa_K1104245])]] |
| − | [[File: NYMU_AA.png|frame|center]] | + | [[File: NYMU_AA.png|frame|center|Promoter testing of ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) and its improvements.]] |
==Discussion== | ==Discussion== | ||
Revision as of 11:46, 3 October 2013
This part: AhpCp2 is one of the improvement versions of BioBrick Part: ahpC promoter (Part:K362001) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCp2 (Part:BBa_K1104205) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].
We use the device: AhpCp2 +E0840 (Part:BBa_K1104245) includes promoter (Part:BBa_K1104205) and Part:BBa_E0840, induce the promoter by different concentrations of H2O2, and identify the strength. After AhpCp2 (Part:BBa_K1104205) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.
We tested the improved promoter: AhpCp2 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing.
Results
Here is our result of AhpCp2 testing:
Discussion
Applications of BBa_K1104205
User Reviews
UNIQ6e8666b6b9375485-partinfo-00000000-QINU UNIQ6e8666b6b9375485-partinfo-00000001-QINU