Difference between revisions of "Part:BBa K1104204:Experience"
| Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
| − | We tested the improved promoter: AhpCp2D1 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. | + | This part: AhpCp2D1 is one of the improvement versions of BioBrick Part: ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCp2D1 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing ''Nosema ceranae'']. |
| + | |||
| + | [[File: NYMU_44.png|frame|center|'''AhpCp1000 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104244 Part:BBa_K1104244])''']] | ||
| + | |||
| + | We use the device: AhpCp2D1 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104244 Part:BBa_K1104244]) includes promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induce the promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identify the strength. After AhpCp1000 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104204 Part:BBa_K1104204]) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP. | ||
| + | |||
| + | We tested the improved promoter: AhpCp2D1 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing. | ||
| + | |||
==Results== | ==Results== | ||
Here is our result of AhpCp2D1 testing: | Here is our result of AhpCp2D1 testing: | ||
[[File: NYMU_AhpCp2D1_strength.png|frame|center]] | [[File: NYMU_AhpCp2D1_strength.png|frame|center]] | ||
| − | [[File: NYMU_all1.png|frame|center]] | + | [[File: NYMU_all1.png|frame|center|Comparison between ahpC and its improvements. ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104244 Part:BBa_K1104244])]] |
| − | [[File: NYMU_AA.png|frame|center]] | + | [[File: NYMU_AA.png|frame|center|Promoter testing of ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) and its improvements.]] |
| + | |||
==Discussion== | ==Discussion== | ||
Revision as of 11:35, 3 October 2013
This part: AhpCp2D1 is one of the improvement versions of BioBrick Part: ahpC promoter (Part:K362001) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCp2D1 (Part:BBa_K1104204) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].
We use the device: AhpCp2D1 +E0840 (Part:BBa_K1104244) includes promoter (Part:BBa_K1104204) and Part:BBa_E0840, induce the promoter by different concentrations of H2O2, and identify the strength. After AhpCp1000 (Part:BBa_K1104204) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.
We tested the improved promoter: AhpCp2D1 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing.
Results
Here is our result of AhpCp2D1 testing:
Discussion
Applications of BBa_K1104204
User Reviews
UNIQ6762574fda1f3a12-partinfo-00000000-QINU UNIQ6762574fda1f3a12-partinfo-00000001-QINU