Difference between revisions of "Part:BBa K1022114"

Revision as of 09:59, 3 October 2013

pT7 : Lysis Device

This bio-brick codes for the promoter pT7(BBa_I712074) followed by the lysis device(BBa_K112808).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1404
Illegal NheI site found at 1427
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1009
Illegal AgeI site found at 1079
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1660

Characterization

For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch TU Delft iGEM13 Wiki !!]

The construct is made by ligating the pT7 promoter BBa_I712074 in front of the lysis device from the biobrick BBa_K112808. This is analyze how the lysis device can be controlled if needed.

Description : The E.coli cells transformed with pT7 lysis cassette BBa_K1022114 is grown on a plate reader which is capable of shaking and heating to 37˚C to take readings of the cells in exponential phase at every 10 minutes. Different range of IPTG concentration is used to characterize the bio – brick BBa_K1022114. At time point, 160 minutes, IPTG is added according to the table below.

LB(µL)	90	94	93	92	91	90	89	88	87	86	85	95
Example	Example	Example
Example	Example	Example

	No cells + 1mM 	0.1mM 	0.2m 	0.3mM 	0.4mM 	0.5mM 	0.6mM 	0.7mM 	0.8mM 	0.9mM 	1mM 	Cell + No IPTG

LB(µL) 90 94 93 92 91 90 89 88 87 86 85 95 Cells(µL) - 5 5 5 5 5 5 5 5 5 5 5 10X IPTG(µL) 10 1 2 3 4 5 6 7 8 9 10 -

@@ Line 21: / Line 21: @@
 Description :
 The <i>E.coli</i> cells transformed with pT7 lysis cassette [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022114  BBa_K1022114] is grown on a plate reader which is capable of shaking and heating to 37˚C to take readings of the cells in exponential phase at every 10 minutes. Different range of IPTG concentration is used to characterize the bio – brick  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022114  BBa_K1022114].  At time point, 160 minutes, IPTG is added according to the table below.
+{| class="wikitable"
+|- No cells + 1mM 0.1mM 0.2m 0.3mM 0.4mM 0.5mM 	0.6mM 0.7mM 0.8mM 0.9mM 1mM Cell + No IPTG
+|-
+| LB(µL) 	|| 90 	|| 94 ||93 	 ||92 	 ||91 	 ||90 	 ||89 ||88 	||87 	 ||86  ||	85 ||	95 ||
+|-
+| Example || Example || Example
+|-
+| Example || Example || Example
+|}
   	No cells + 1mM 	0.1mM 	0.2m 	0.3mM 	0.4mM 	0.5mM 	0.6mM 	0.7mM 	0.8mM 	0.9mM 	1mM 	Cell + No IPTG