Difference between revisions of "Part:BBa K1022114"
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The construct is made by ligating the pT7 promoter [https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074] in front of the lysis device from the biobrick [https://parts.igem.org/wiki/index.php?title=Part:BBa_K112808 BBa_K112808]. This is analyze how the lysis device can be controlled if needed. | The construct is made by ligating the pT7 promoter [https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074] in front of the lysis device from the biobrick [https://parts.igem.org/wiki/index.php?title=Part:BBa_K112808 BBa_K112808]. This is analyze how the lysis device can be controlled if needed. | ||
| + | [[Image:114.jpg|450px|center]] | ||
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| + | Description : | ||
| + | The <i>E.coli</i> cells transformed with pT7 lysis cassette [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022114 BBa_K1022114] is grown on a plate reader which is capable of shaking and heating to 37˚C to take readings of the cells in exponential phase at every 10 minutes. Different range of IPTG concentration is used to characterize the bio – brick [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022114 BBa_K1022114]. At time point, 160 minutes, IPTG is added according to the table below. | ||
[[Image:Lysis TU Delft.jpg|450px|center]] | [[Image:Lysis TU Delft.jpg|450px|center]] | ||
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Revision as of 09:43, 3 October 2013
pT7 : Lysis Device
This bio-brick codes for the promoter pT7(BBa_I712074) followed by the lysis device(BBa_K112808).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1404
Illegal NheI site found at 1427 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1009
Illegal AgeI site found at 1079 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1660
Characterization
For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch TU Delft iGEM13 Wiki !!]
The construct is made by ligating the pT7 promoter BBa_I712074 in front of the lysis device from the biobrick BBa_K112808. This is analyze how the lysis device can be controlled if needed.
Description : The E.coli cells transformed with pT7 lysis cassette BBa_K1022114 is grown on a plate reader which is capable of shaking and heating to 37˚C to take readings of the cells in exponential phase at every 10 minutes. Different range of IPTG concentration is used to characterize the bio – brick BBa_K1022114. At time point, 160 minutes, IPTG is added according to the table below.
