Difference between revisions of "Part:BBa K1022114"
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<partinfo>BBa_K1022114 short</partinfo> | <partinfo>BBa_K1022114 short</partinfo> | ||
− | This bio-brick codes for the promoter pT7 followed by the lysis device. | + | This bio-brick codes for the promoter pT7(BBa_I712074) followed by the lysis device(BBa_K112808). |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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− | === | + | ===Characterization=== |
+ | '''For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch!!]''' | ||
+ | |||
+ | The construct is made by ligating the pT7 promoter(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074" target="_blank">BBa_I712074</a>) in front of the lysis device from the biobrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K112808" target="_blank"> BBa_K112808</a>. This is analyze how the lysis device can be controlled if needed. | ||
+ | |||
+ | <center> | ||
+ | <a href="https://static.igem.org/mediawiki/2013/c/c0/114.jpg" target="_blank"><img src="https://static.igem.org/mediawiki/2013/c/c0/114.jpg"/></a> | ||
+ | </center> | ||
+ | <center> | ||
+ | <p>Figure 2: Part BBa_K1022114</p></div></center> | ||
+ | <br> | ||
+ | |||
+ | |||
+ | |||
Revision as of 08:14, 3 October 2013
pT7 : Lysis Device
This bio-brick codes for the promoter pT7(BBa_I712074) followed by the lysis device(BBa_K112808).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1404
Illegal NheI site found at 1427 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1009
Illegal AgeI site found at 1079 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1660
Characterization
For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch!!]
The construct is made by ligating the pT7 promoter(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074" target="_blank">BBa_I712074</a>) in front of the lysis device from the biobrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K112808" target="_blank"> BBa_K112808</a>. This is analyze how the lysis device can be controlled if needed.
<a href="" target="_blank"><img src=""/></a>
Figure 2: Part BBa_K1022114
</div>