Difference between revisions of "Part:BBa K1104202:Experience"
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
  
−
This part HemHp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104201 Part:BBa_K1104201]) was used as sensor to ROS(Reactive Oxygen Species). More details about ROS-induced promoters are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].
+
HemHp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104201 Part:BBa_K1104201]) is an OxyR-induced promoter, and was used as sensor to ROS(Reactive Oxygen Species).
  
−
We use the device: HemH promoter +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104242 Part:BBa_K1104242]) includes promoter HemHp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K110424 Part:BBa_K1104242]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 BBa_E0840], induce the promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identify the strength.
+
[[File: NYMU_42.png|frame|center|'''HemH promoter +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104242 Part:BBa_K1104242])''']]
  
−
[[File: NYMU_42.png|frame|center|'''HemH promoter +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104241 Part:BBa_K1104242])''']]
+
We designed the device: HemH promoter +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104242 Part:BBa_K1104242]) includes HemH promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104242 Part:BBa_K1104242]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induced HemH promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identified the strength.
  
−
After HemHp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104201 Part:BBa_K1104201]) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.
+
In this device, after HemHp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104201 Part:BBa_K1104201]) is activated, GFP generator [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840] takes as input a transcriptional signal (PoPS) and produce as output GFP (green fluorescent protein). We induced HemH promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and measured the expression of GFP, and identified the strength.  
  
 +
More details about ROS-induced promoters are in page: [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing ''Nosema ceranae''].
  
 
==Results==
 
==Results==

Revision as of 07:17, 3 October 2013


HemHp (Part:BBa_K1104201) is an OxyR-induced promoter, and was used as sensor to ROS(Reactive Oxygen Species).

HemH promoter +E0840 (Part:BBa_K1104242)

We designed the device: HemH promoter +E0840 (Part:BBa_K1104242) includes HemH promoter (Part:BBa_K1104242) and Part:BBa_E0840, induced HemH promoter by different concentrations of H2O2, and identified the strength.

In this device, after HemHp (Part:BBa_K1104201) is activated, GFP generator Part:BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output GFP (green fluorescent protein). We induced HemH promoter by different concentrations of H2O2, and measured the expression of GFP, and identified the strength.

More details about ROS-induced promoters are in page: [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].

Results

Discussion

Applications of BBa_K1104202

User Reviews

UNIQef9e1d8dfa8f0642-partinfo-00000000-QINU UNIQef9e1d8dfa8f0642-partinfo-00000001-QINU