Difference between revisions of "Part:BBa K1051204"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1051204 short</partinfo>
 
<partinfo>BBa_K1051204 short</partinfo>
  
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PEST
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<h3>Purpose</h3>
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Works for the degradation of fluorescent proteins we used at C-terminal.
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<br />
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<h3>Principle</h3>
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'''PEST''',Certain amino acid sequences appear to be signals for degradation. One such sequence is known as the PEST sequence because short stretch of about eight amino acids is enriched with proline(P), glutamic acid(E), serine(S), and threonine(T) at C-terminal of proteins that are more rapidly degraded than other proteins.
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<br />
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<h3>Engineering</h3>
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We convert base "A" to "G" at 6 site of cln3-PEST by designing primer.
  
 
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<span class='h3bb'>Sequence and Features</span>
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<h3>Sequence and Features</h3>
 
<partinfo>BBa_K1051204 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1051204 SequenceAndFeatures</partinfo>
  
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<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1051204 parameters</partinfo>
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<partinfo>BBa_K1051202 parameters</partinfo>
 
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<h3>References</h3>
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[1] Li X, Zhao X, Fang Y, Jiang X, Duong T, Fan C, Huang CC, Kain SR(1998) Generation of destabilized green fluorescent protein as a transcription reporter. J Biol Chem 273:34970–34975. <br />
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[2] Dorota Skowyra, Karen L Craig, Mike Tyers, Stephen J Elledge, J.Wade Harper "F-Box Proteins Are Receptors that Recruit Phosphorylated Substrates to the SCF Ubiquitin-Ligase Complex".

Latest revision as of 03:14, 3 October 2013

cln3-PEST

Purpose

Works for the degradation of fluorescent proteins we used at C-terminal.

Principle

PEST,Certain amino acid sequences appear to be signals for degradation. One such sequence is known as the PEST sequence because short stretch of about eight amino acids is enriched with proline(P), glutamic acid(E), serine(S), and threonine(T) at C-terminal of proteins that are more rapidly degraded than other proteins.

Engineering

We convert base "A" to "G" at 6 site of cln3-PEST by designing primer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Li X, Zhao X, Fang Y, Jiang X, Duong T, Fan C, Huang CC, Kain SR(1998) Generation of destabilized green fluorescent protein as a transcription reporter. J Biol Chem 273:34970–34975.
[2] Dorota Skowyra, Karen L Craig, Mike Tyers, Stephen J Elledge, J.Wade Harper "F-Box Proteins Are Receptors that Recruit Phosphorylated Substrates to the SCF Ubiquitin-Ligase Complex".