Difference between revisions of "Part:BBa K1149004"

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https://static.igem.org/mediawiki/2013/thumb/c/c1/PueB.png/450px-PueB.png
 
https://static.igem.org/mediawiki/2013/thumb/c/c1/PueB.png/450px-PueB.png
  
PueB growth assay. E. coli (MG1655) transformed with PueB BBa_K1149004 were grown with either 0% or 2% Xylose to induce PueB expression. There was no difference between no induction and induction as a t-test gave p = 0.6040 > 0.05. Growth was at 37°C with shaking. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM.
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'''PueB growth assay'''. ''E. coli'' (MG1655) transformed with PueB BBa_K1149004 were grown with either 0% or 2% Xylose to induce PueB expression. There was no difference between no induction and induction as a t-test gave p = 0.6040 > 0.05. Growth was at 37°C with shaking. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM.
  
 
<b>Conclusion: There is no growth inhibition caused by induction in PueB. </b>
 
<b>Conclusion: There is no growth inhibition caused by induction in PueB. </b>

Revision as of 22:20, 2 October 2013

pelB-pueB expression

PUR degradation enzyme from Pseudomonas chlororaphis.

PUR_esterase_theoretical_pathway_2.jpg

Characterisation

Our PueB construct was tested for growth upon induction by addition of Xylose.

450px-PueB.png

PueB growth assay. E. coli (MG1655) transformed with PueB BBa_K1149004 were grown with either 0% or 2% Xylose to induce PueB expression. There was no difference between no induction and induction as a t-test gave p = 0.6040 > 0.05. Growth was at 37°C with shaking. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM.

Conclusion: There is no growth inhibition caused by induction in PueB.

References:

[http://www.sciencedirect.com/science/article/pii/S0964830501000427 Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis (2001)]

[http://www.sciencedirect.com/science/article/pii/S0964830502000690 secretion and expression in E.coli]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BglII site found at 1213
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 407
    Illegal AgeI site found at 752
    Illegal AgeI site found at 1241
    Illegal AgeI site found at 1517
  • 1000
    COMPATIBLE WITH RFC[1000]