Difference between revisions of "Part:BBa K1111013"

(→‎Introduction)
(→‎Usage and Biology)
Line 12: Line 12:
  
  
−
<span class='h3bb'>Sequence and Features</span>
+
==<span class='h3bb'>Sequence and Features</span>==
 
<partinfo>BBa_K1111013 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1111013 SequenceAndFeatures</partinfo>
  

Revision as of 21:23, 2 October 2013

Ice Nucleation Protein fused to Streptavidin Dead

Introduction

It is a fusion protein between an Ice Nucleation Protein (INP) and Streptavidin Alive made by Gibson Assembly. INP is an outer membrane protein found in the genome of Pseudomonas syringae, that is recognize by the E.coli protein secretion machinery.

Usage and Biology

This part was designed to express streptavidin on the outer membrane of E.coli.
It serves as a negative control as the dead Streptavidin is though to not bind to biotin or with really decreased affinity.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1727
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1727
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1727
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 1691
    Illegal AgeI site found at 1742
  • 1000
    COMPATIBLE WITH RFC[1000]