Difference between revisions of "Part:BBa K1151001:Design"

(Source)
(Design Notes)
 
(2 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
The gene is designed on H.pylori genomic sequence and amplified by PCR.
+
The gene is designed on ''H.pylori'' genomic sequence.
  
 
===Source===
 
===Source===
Line 14: Line 14:
  
 
===References===
 
===References===
 +
 +
1.  Gilbert  et al,  Protein Hpn: cloning and characterization of a Histidine-Rich Metal-Binding Polypeptide in  Helicobacter pylori  and  Helicobacter mustelae.  Infect Immun. 1995 Jul; 63(7):2682-8.
 +
 +
2.  Ruiguang et al,  Expression and characterization of a histidine-rich protein, Hpn: potential for Ni2+ storage in Helicobacter pylori. Biochem J. 2006 Jan 1;393 (Pt 1): 285-93.
 +
 +
3.  Seshadri et al, Roles of His-Rich Hpn and Hpn-Like Proteins in Helicobacter pylori Nickel Physiology. J Bacteriol. 2007 Jun;189(11):4120-6. Epub 2007 Mar 23.

Latest revision as of 19:17, 2 October 2013


Histidine-rich metal-binding protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene is designed on H.pylori genomic sequence.

Source

Synthesized by IDT (as Gblock).

References

1. Gilbert et al, Protein Hpn: cloning and characterization of a Histidine-Rich Metal-Binding Polypeptide in Helicobacter pylori and Helicobacter mustelae. Infect Immun. 1995 Jul; 63(7):2682-8.

2. Ruiguang et al, Expression and characterization of a histidine-rich protein, Hpn: potential for Ni2+ storage in Helicobacter pylori. Biochem J. 2006 Jan 1;393 (Pt 1): 285-93.

3. Seshadri et al, Roles of His-Rich Hpn and Hpn-Like Proteins in Helicobacter pylori Nickel Physiology. J Bacteriol. 2007 Jun;189(11):4120-6. Epub 2007 Mar 23.