Difference between revisions of "Part:BBa K1111002:Experience"

(→‎Gibson Assembly)
(→‎Gibson Assembly)
Line 9: Line 9:
 
===Gibson Assembly===
 
===Gibson Assembly===
 
We used Gibson Assembly to insert the Cad promoter into the pSB1C3 Backbone. We used the biobrick BBa_I746908 where we remove the Arac promoter but left the superfolded GFP. This way, the latter was under control of our Cad promoter.
 
We used Gibson Assembly to insert the Cad promoter into the pSB1C3 Backbone. We used the biobrick BBa_I746908 where we remove the Arac promoter but left the superfolded GFP. This way, the latter was under control of our Cad promoter.
 +
 +
[[Image: Team-EPF-Lausanne 1.1 History.jpg|thumb|600px|center|Figure 1: History of the Gibson Assembly including Primers]]
  
 
===Sequencing===
 
===Sequencing===

Revision as of 17:47, 2 October 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1111002

We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.

Gibson Assembly

We used Gibson Assembly to insert the Cad promoter into the pSB1C3 Backbone. We used the biobrick BBa_I746908 where we remove the Arac promoter but left the superfolded GFP. This way, the latter was under control of our Cad promoter.

Figure 1: History of the Gibson Assembly including Primers

Sequencing

Figure 1:

User Reviews

UNIQ2c63244fc2d763fa-partinfo-00000000-QINU UNIQ2c63244fc2d763fa-partinfo-00000001-QINU