Difference between revisions of "Part:BBa K1132018"
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This biobrick was created by assembling two preexisting biobricks: a gene encoding a mutated version of the red fluorescent protein (BBa_K081014) with rbs and terminator and put under the control of FixK2 (BBa_K592006), a blue light sensitive promoter (465-482nm). FixK2 was previously described by iGEM11_Uppsala-Sweden as working with FixJ. | This biobrick was created by assembling two preexisting biobricks: a gene encoding a mutated version of the red fluorescent protein (BBa_K081014) with rbs and terminator and put under the control of FixK2 (BBa_K592006), a blue light sensitive promoter (465-482nm). FixK2 was previously described by iGEM11_Uppsala-Sweden as working with FixJ. | ||
<br>When FixJ is phosphorylated, it can bind FixK2 and thus activate the downstream system. | <br>When FixJ is phosphorylated, it can bind FixK2 and thus activate the downstream system. | ||
| − | <br>In the litterature (Andreas Möglich et al. 2009. Design and Signaling Mechanism of Light-Regulated Histidine Kinases.), FixJ is phosphorylated by YF1 in darkness and unphosphorylated in light. BBa_K592016 is a composite part using YF1 with FixJ. | + | <br>In the litterature (Andreas Möglich et al. 2009. Design and Signaling Mechanism of Light-Regulated Histidine Kinases.), FixJ is phosphorylated by YF1 in darkness and unphosphorylated in light. BBa_K592016 is a composite part using YF1 with FixJ.<br> |
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https://static.igem.org/mediawiki/2013/a/ad/2018_-_copie.png | https://static.igem.org/mediawiki/2013/a/ad/2018_-_copie.png | ||
| − | <!-- Add more about the biology of this part here | + | <br> Alexander Deiters. Light Activation as a Method of Regulating and Studying Gene Expression. 2009<!-- Add more about the biology of this part here |
===Usage and Biology=== | ===Usage and Biology=== | ||
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| − | <span class='h3bb'> | + | <span class='h3bb'></span> |
<partinfo>BBa_K1132018 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1132018 SequenceAndFeatures</partinfo> | ||
Latest revision as of 16:03, 2 October 2013
FixK2 promoter-rbs-mRFP-term
This biobrick was created by assembling two preexisting biobricks: a gene encoding a mutated version of the red fluorescent protein (BBa_K081014) with rbs and terminator and put under the control of FixK2 (BBa_K592006), a blue light sensitive promoter (465-482nm). FixK2 was previously described by iGEM11_Uppsala-Sweden as working with FixJ.
When FixJ is phosphorylated, it can bind FixK2 and thus activate the downstream system.
In the litterature (Andreas Möglich et al. 2009. Design and Signaling Mechanism of Light-Regulated Histidine Kinases.), FixJ is phosphorylated by YF1 in darkness and unphosphorylated in light. BBa_K592016 is a composite part using YF1 with FixJ.
Alexander Deiters. Light Activation as a Method of Regulating and Studying Gene Expression. 2009
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 834
Illegal AgeI site found at 946 - 1000COMPATIBLE WITH RFC[1000]