Difference between revisions of "Part:BBa K1132018"

 
 
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<partinfo>BBa_K1132018 short</partinfo>
 
<partinfo>BBa_K1132018 short</partinfo>
  
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This biobrick was created by assembling two preexisting biobricks: a gene encoding a mutated version of the red fluorescent protein (BBa_K081014) with rbs and terminator and put under the control of  FixK2  (BBa_K592006), a blue light sensitive promoter (465-482nm). FixK2 was previously described by iGEM11_Uppsala-Sweden as working with FixJ.
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<br>When FixJ is phosphorylated, it can bind FixK2 and thus activate the downstream system.
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<br>In the litterature (Andreas Möglich et al. 2009. Design and Signaling Mechanism of Light-Regulated Histidine Kinases.), FixJ is phosphorylated by YF1 in darkness and unphosphorylated in light. BBa_K592016 is a composite part using YF1 with FixJ.<br>
  
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https://static.igem.org/mediawiki/2013/a/ad/2018_-_copie.png
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<br> Alexander Deiters. Light Activation as a Method of Regulating and Studying Gene Expression. 2009<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1132018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1132018 SequenceAndFeatures</partinfo>
  

Latest revision as of 16:03, 2 October 2013

FixK2 promoter-rbs-mRFP-term

This biobrick was created by assembling two preexisting biobricks: a gene encoding a mutated version of the red fluorescent protein (BBa_K081014) with rbs and terminator and put under the control of FixK2 (BBa_K592006), a blue light sensitive promoter (465-482nm). FixK2 was previously described by iGEM11_Uppsala-Sweden as working with FixJ.
When FixJ is phosphorylated, it can bind FixK2 and thus activate the downstream system.
In the litterature (Andreas Möglich et al. 2009. Design and Signaling Mechanism of Light-Regulated Histidine Kinases.), FixJ is phosphorylated by YF1 in darkness and unphosphorylated in light. BBa_K592016 is a composite part using YF1 with FixJ.

2018_-_copie.png
Alexander Deiters. Light Activation as a Method of Regulating and Studying Gene Expression. 2009


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 834
    Illegal AgeI site found at 946
  • 1000
    COMPATIBLE WITH RFC[1000]