Difference between revisions of "Part:BBa K1151005"
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Once inserted the gene coding for pUreA in a PSB1C3 plasmid (digestion and ligation), we transformed DH5a cells with the construct and then we recovered the ligated plasmid by boiling prep. We then proceeded with the digestion with EcoRI and PstI to check. | Once inserted the gene coding for pUreA in a PSB1C3 plasmid (digestion and ligation), we transformed DH5a cells with the construct and then we recovered the ligated plasmid by boiling prep. We then proceeded with the digestion with EcoRI and PstI to check. | ||
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| − | Figure 1: | + | [[File:boiling1Unisalento.jpg]] |
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| + | '''Figure 1:''' Digestion result of boiling prep plasmids. | ||
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| + | ===PCR with VF2 and VR2 primers=== | ||
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| + | [[File:VF22Unisalento.jpg]] | ||
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| + | '''Figure 2:''' PCR results. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 14:45, 2 October 2013
pUreA
This promoter derived from ureAB operon of Helicobacter pylori. It can be linked by HpNikR (BBa_K1151000) in nickel presence for promoting trascription of the downstream gene.
Boiling prep and digestion with restriction enzymes
Once inserted the gene coding for pUreA in a PSB1C3 plasmid (digestion and ligation), we transformed DH5a cells with the construct and then we recovered the ligated plasmid by boiling prep. We then proceeded with the digestion with EcoRI and PstI to check.
Figure 1: Digestion result of boiling prep plasmids.
PCR with VF2 and VR2 primers
Figure 2: PCR results.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]