Difference between revisions of "Part:BBa K1150044"

Revision as of 13:19, 2 October 2013

uniCAS RNaimer multiple targets to Bla target 3 and 4

uniCAS RNaimer multiple targets to Bla target 3 and 4
Function	twofold targeting of the bla-gen with one plasmid
Use in	Mammalian cells
RFC standard	RFC 25
Backbone	pSB1C3
Submitted by	[http://2013.igem.org/Team:Freiburg Freiburg 2013]

Usage and Biology

Fusion of bla-target 3 and bla-target 4. This RNAimer was build via iGEM-ligation method and contains the DNA-target sequences of two areas of the bla-gene. This enables multiple gene targeting throught one plasmid. The transfection with such a multiple target-plasmid avoids additional cell stress compared to transfection with serveral single target-plasmids.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 224
Illegal BglII site found at 1061
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 216
Illegal BsaI.rc site found at 1053

@@ Line 22: / Line 22: @@
 ===Usage and Biology===
-Fusion of [https://parts.igem.org/Part:BBa_K1150041 Target 3] and [https://parts.igem.org/Part:BBa_K1150042 Target 4].
+Fusion of [https://parts.igem.org/Part:BBa_K1150041 bla-target 3] and [https://parts.igem.org/Part:BBa_K1150042 bla-target 4].
 This RNAimer was build via iGEM-ligation method and contains the DNA-target sequences of two areas of the bla-gene. This enables multiple gene targeting throught one plasmid. The transfection with such a multiple target-plasmid avoids additional cell stress compared to transfection with serveral single target-plasmids.