Difference between revisions of "Part:BBa K1151010"
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This is a nickel sensitive system driven by the promoter pexbB ([[BBa_K1151004]]) with a GFP reporter. Normally the production of GFP is switched on, it is possible to repress the GFP expression using nickel ions in medium and in presence of HpNikR ([[BBa_K1151000]]) as trascription factor.
 
This is a nickel sensitive system driven by the promoter pexbB ([[BBa_K1151004]]) with a GFP reporter. Normally the production of GFP is switched on, it is possible to repress the GFP expression using nickel ions in medium and in presence of HpNikR ([[BBa_K1151000]]) as trascription factor.
  
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===Boiling prep and digestion with restriction enzymes===
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===Usage and Biology===
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              [[File:EPEP.jpg]]
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'''Figure 1:''' Digestion result of boiling prep plasmids.
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===PCR with VF2 and VR2 primers===
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[[File:PCR33.jpg]]
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'''Figure 2:''' PCR results.
 
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===Sequence and Features===
 
===Sequence and Features===

Revision as of 11:45, 2 October 2013

GFP nickel regulated 2

This is a nickel sensitive system driven by the promoter pexbB (BBa_K1151004) with a GFP reporter. Normally the production of GFP is switched on, it is possible to repress the GFP expression using nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor.

Boiling prep and digestion with restriction enzymes

             EPEP.jpg

Figure 1: Digestion result of boiling prep plasmids.

PCR with VF2 and VR2 primers

PCR33.jpg

Figure 2: PCR results.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 830