Difference between revisions of "Part:BBa K1151009"

Revision as of 11:33, 2 October 2013

GFP nickel regulated 1

This is a nickel sensitive system driven by the promoter pnikR (BBa_K1151003) with a GFP reporter. Normally the production of GFP is switched on, it is possible to repress the GFP expression using nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor.

Boiling prep and digestion with restriction enzymes

Once inserted the construct in a PSB1C3 plasmid (digestion and ligation), we transformed DH5a cells and then we recovered the ligated plasmid by boiling prep. We then proceeded with the digestion with EcoRI and PstI to check.

Figure 1: Digestion result of boiling prep plasmids.

PCR with VF2 and VR2 primers

Figure 2: PCR results.

Sequence and Features