Difference between revisions of "Part:BBa K1111013:Experience"
| Line 3: | Line 3: | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | + | ==Applications of BBa_K1111013== | |
| + | As said in the main page, this part is a negative control with a lower Kd compared to BBa_K1111012 and BBa_K1111014. | ||
| − | + | ==Sequencing== | |
We sequenced this part once the Gibson assembly made. To do so, we used primers for iGEM sites VF2 and VR, in order to sequence all that was inserted in the backbone pSB1C3. | We sequenced this part once the Gibson assembly made. To do so, we used primers for iGEM sites VF2 and VR, in order to sequence all that was inserted in the backbone pSB1C3. | ||
The sequencing results are shown here : | The sequencing results are shown here : | ||
| − | + | ==Microscopy== | |
Also, we transformed cells and used them to do microscopy. Two experiments were done, one with a fluorescent antibody against streptavidin and the other one with a fluorescent biotin. We did both in case the antibody would be too big, since biotin is a small molecule best suit to cross glycocalix. | Also, we transformed cells and used them to do microscopy. Two experiments were done, one with a fluorescent antibody against streptavidin and the other one with a fluorescent biotin. We did both in case the antibody would be too big, since biotin is a small molecule best suit to cross glycocalix. | ||
Revision as of 10:50, 2 October 2013
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how you used this part and how it worked out.
Applications of BBa_K1111013
As said in the main page, this part is a negative control with a lower Kd compared to BBa_K1111012 and BBa_K1111014.
Sequencing
We sequenced this part once the Gibson assembly made. To do so, we used primers for iGEM sites VF2 and VR, in order to sequence all that was inserted in the backbone pSB1C3. The sequencing results are shown here :
Microscopy
Also, we transformed cells and used them to do microscopy. Two experiments were done, one with a fluorescent antibody against streptavidin and the other one with a fluorescent biotin. We did both in case the antibody would be too big, since biotin is a small molecule best suit to cross glycocalix.
User Reviews
UNIQ5c1561179bf9d3d7-partinfo-00000000-QINU UNIQ5c1561179bf9d3d7-partinfo-00000001-QINU