Difference between revisions of "Part:BBa K1151001"
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===Digestion with restriction enzymes=== | ===Digestion with restriction enzymes=== | ||
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===Sequence and Features=== | ===Sequence and Features=== | ||
<partinfo>BBa_K1151001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1151001 SequenceAndFeatures</partinfo> | ||
Revision as of 09:52, 2 October 2013
Histidine-rich metal-binding protein
So called for emphasize its origins in Helicobacter pylori and its avidity for nickel. Consisting of 60 aminoacids, the protein is rich in histidine (28 residues, 46.7 %) and contains short repeating motifs, it exists as an equilibration of multimeric forms in solution, with 20-mers (approx. 136 kDa) being the predominant species. Hpn can bind tightly and reversibly up to five Ni2+ ions per each monomer of 7 kDa in a pH-dependent manner (pH 7.4 ). In H. pylori play an important role in storing nickel required to the survival of the bacterium.
Digestion with restriction enzymes
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]