Difference between revisions of "Part:BBa K1075020"
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The mutation ssrA (DAS+4) prevents a direct binding to the protease and ensures the dependance of sspB.
 
The mutation ssrA (DAS+4) prevents a direct binding to the protease and ensures the dependance of sspB.
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1075022 SequenceAndFeatures</partinfo>
  
 
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Revision as of 20:57, 1 October 2013

E. coli ssrA(DAS+4)

fused to the C terminus of a protein it triggers degradation upon induction of sspB function.


Usage and Biology

The ssrA tag is a sequence, which allows proteolytic enzymes to degrade them. It relates to the protease ClpXP complex in E.coli and it also allows adaptor proteins such as sspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutation ssrA (DAS+4) prevents a direct binding to the protease and ensures the dependance of sspB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 762
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]