Difference between revisions of "Part:BBa K1189000"
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<img src="https://static.igem.org/mediawiki/parts/4/4d/YYC2013_TALE_Construct_His_TALE_A_White_Background.jpg">
 
<img src="https://static.igem.org/mediawiki/parts/4/4d/YYC2013_TALE_Construct_His_TALE_A_White_Background.jpg">
 
</figure>
 
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<figcaption>Fig 1: Shows the repeat variable domain(RVD) of TALEs. These RVDs are very specific and the code of 2 amino acids that bind to each nucleotide has been solved making TALEs very easy to engineer and very specific to their targets</figcaption>
 
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Revision as of 20:07, 1 October 2013

TALE-A with a his tag under a lacI promoter

This part was built to allow for the extraction of TALA proteins with the his-tags. This part was also built with J04500, an IPTG inducible promoter with RBS. TALA proteins can specifically bind to DNA.

Fig 1: Shows the repeat variable domain(RVD) of TALEs. These RVDs are very specific and the code of 2 amino acids that bind to each nucleotide has been solved making TALEs very easy to engineer and very specific to their targets

Calgary2013-TALE-RVD.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]