Difference between revisions of "Part:BBa K1045017"

 
 
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<partinfo>BBa_K1045017 short</partinfo>
 
<partinfo>BBa_K1045017 short</partinfo>
  
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This composite part encodes a reporter system to monitor the repression mediated by DarR. DarR is a transcriptional repressor that binds to the DarR operator sequence blocking transcription of the downstream gene. These effects are enhanced by c-di-AMP. In part [[Part:BBa_K1045017|BBa_K1045017]], the DarR operator is placed in between a strong promoter [[Part:BBa_J23110|BBa_J23110]] and a GFP generator [[Part:BBa_E0240|BBa_E0240]]. DarR itself is part of an inverted expression unit with a weak promoter [[Part:BBa_J23117|BBa_J23117]]. Transcription of the DarR mRNA might be terminated by an inverse terminator [[Part:BBa_K1045009|BBa_K1045009]]. This system was designed to screen for compounds similar to c-di-AMP.
  
 
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Latest revision as of 19:39, 1 October 2013

DarR reporter system

This composite part encodes a reporter system to monitor the repression mediated by DarR. DarR is a transcriptional repressor that binds to the DarR operator sequence blocking transcription of the downstream gene. These effects are enhanced by c-di-AMP. In part BBa_K1045017, the DarR operator is placed in between a strong promoter BBa_J23110 and a GFP generator BBa_E0240. DarR itself is part of an inverted expression unit with a weak promoter BBa_J23117. Transcription of the DarR mRNA might be terminated by an inverse terminator BBa_K1045009. This system was designed to screen for compounds similar to c-di-AMP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 806
    Illegal NheI site found at 829
    Illegal NheI site found at 909
    Illegal NheI site found at 932
    Illegal NotI site found at 718
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 731
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 860
    Illegal AgeI site found at 168
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1630