Difference between revisions of "Part:BBa K1033000:Design"

 
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1033000 short</partinfo>
 
<partinfo>BBa_K1033000 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Earlier in iGEM eucaryot TAL has been used, however we used bacterial TAL for better compatibility with E.coli.
+
Earlier in iGEM eukaryot TAL has been used, however we used bacterial TAL for better compatibility with bacteria.  
  
  
Line 16: Line 15:
  
 
===References===
 
===References===
 +
[1].Zhixiong Xue, Michael McCluskey, Keith Cantera, F. Sima Sariaslani, Lixuan Huang (2007) Identification, characterization and functional expression of a tyrosine ammonia-lyase and its mutants from the photosynthetic bacterium Rhodobacter sphaeroides. J Ind Microbiol Biotechnol 34:599-604
 +
 +
[2]. J.A. Kyndt, T.E. Meyer, M.A Cusanovich, J.J. Van Beeumen (2002) Characterization of a bacterial tyrosine ammonia lyase, a biosynthetic enzyme for the photoactive yellow protein. FEBS Letters 512 240-244
 +
 +
[3]. Robert J. Conrado et al, DNA guided assembly of biosynthetic pathways promotes improved catalytic effiency. Nucleic Acids Research , 2012, Vol 40 NO 4, 1879-1889

Latest revision as of 18:57, 1 October 2013

Tyrosine ammonia-lyase (TAL) with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 45
    Illegal NgoMIV site found at 877
    Illegal AgeI site found at 140
    Illegal AgeI site found at 306
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1335


Design Notes

Earlier in iGEM eukaryot TAL has been used, however we used bacterial TAL for better compatibility with bacteria.


Source

Rhodobacter sphaeroides

References

[1].Zhixiong Xue, Michael McCluskey, Keith Cantera, F. Sima Sariaslani, Lixuan Huang (2007) Identification, characterization and functional expression of a tyrosine ammonia-lyase and its mutants from the photosynthetic bacterium Rhodobacter sphaeroides. J Ind Microbiol Biotechnol 34:599-604

[2]. J.A. Kyndt, T.E. Meyer, M.A Cusanovich, J.J. Van Beeumen (2002) Characterization of a bacterial tyrosine ammonia lyase, a biosynthetic enzyme for the photoactive yellow protein. FEBS Letters 512 240-244

[3]. Robert J. Conrado et al, DNA guided assembly of biosynthetic pathways promotes improved catalytic effiency. Nucleic Acids Research , 2012, Vol 40 NO 4, 1879-1889