Difference between revisions of "Part:BBa K1104200"

Revision as of 17:44, 1 October 2013

OxyR

Introduction

OxyR is Escherichia coli transcription factor that senses H₂O₂ to activate transcription, and is activated through the formation of an intramolecular disulfide bond.

OxyR(BBa_K1104200)

Literature Study of OxyR

Illustration of OxyR Activation

OxyR, a positive regulator of hydrogen peroxide-inducible genes in E. coli. The OxyR transcription factor regulates the H₂O₂ response of E. coli. H₂O₂ oxidizes OxyR Cys199 to an R-SOH that reacts with Cys208 in an intramolecular disulphide bond.

Structure of the OxyR Regulatory Domain
(A)Reduced Form: Inactive
(B)Oxidized Form: Activated by ROS

_{Reference: D'Autréaux, B., & Toledano, M. B. (October 01, 2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8, 10, 813-824.}

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 ===Introduction===
-The Escherichia coli OxyR transcription factor senses
+OxyR is Escherichia coli transcription factor that senses
 H<sub>2</sub>O<sub>2</sub> to activate transcription, and is activated through the formation of an intramolecular disulfide bond.
+[[File: NYMU_NYMU_OxyRcod.png|frame|center|'''OxyR([https://parts.igem.org/BBa_K1104200 BBa_K1104200])''']]
+===Literature Study of OxyR===
+[[File: NYMU_OxyRactive.png|frame|center|'''Illustration of OxyR Activation''']]
+OxyR, a positive regulator of hydrogen peroxide-inducible genes in ''E. coli''.
+The OxyR transcription factor regulates the H<sub>2</sub>O<sub>2</sub> response of ''E. coli''. H<sub>2</sub>O<sub>2</sub> oxidizes OxyR Cys199 to an R-SOH that reacts with Cys208 in an intramolecular disulphide bond.
+[[File: NYMU_ORD.png|thumb|300px|center|'''Structure of the OxyR Regulatory Domain'''<br />(A)Reduced Form: Inactive<br />(B)Oxidized Form: Activated by ROS<br /> ]]
+:::::<sub>Reference: D'Autréaux, B., & Toledano, M. B. (October 01, 2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8, 10, 813-824.</sub>
+<!-- Add more about the biology of this part here
+===Usage and Biology===
+==Related Parts==
 There are promoters that can be activated by OxyR: TrxCp([https://parts.igem.org/Part:BBa_K1104201 Part:BBa_K1104201]), HemHp([https://parts.igem.org/Part:BBa_K1104202 Part:BBa_K1104202]), ahpC([https://parts.igem.org/Part:BBa_K362001 Part:BBa_K362001)], sufA([https://parts.igem.org/Part:BBa_K362005 Part:BBa_K362005]).
@@ Line 20: / Line 35: @@
 **AhpCp1([https://parts.igem.org/Part:BBa_K1104207 Part:BBa_K1104207])
 **DsbGp([https://parts.igem.org/Part:BBa_K1104208 Part:BBa_K1104208])
-===Literature Study of OxyR===
-[[File: NYMU_OxyRactive.png|frame|center|'''Illustration of OxyR Activation''']]
-OxyR, a positive regulator of hydrogen peroxide-inducible genes in ''E. coli''.
-The OxyR transcription factor regulates the H<sub>2</sub>O<sub>2</sub> response of ''E. coli''. H<sub>2</sub>O<sub>2</sub> oxidizes OxyR Cys199 to an R-SOH that reacts with Cys208 in an intramolecular disulphide bond.
-[[File: NYMU_ORD.png|thumb|300px|center|'''Structure of the OxyR Regulatory Domain'''<br />(A)Reduced Form: Inactive<br />(B)Oxidized Form: Activated by ROS<br /> ]]
-:::::<sub>Reference: D'Autréaux, B., & Toledano, M. B. (October 01, 2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8, 10, 813-824.</sub>
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->