Difference between revisions of "Part:BBa K1104200"

Revision as of 12:42, 1 October 2013

OxyR

Introduction

The Escherichia coli OxyR transcription factor senses H₂O₂ to activate transcription, and is activated through the formation of an intramolecular disulfide bond.

There are promoters that can be activated by OxyR: TrxCp(Part:BBa_K1104201), HemHp(Part:BBa_K1104202), ahpC, sufA(Part:BBa_K362005)

Literature Study of OxyR

Illustration of OxyR Activation

OxyR, a positive regulator of hydrogen peroxide-inducible genes in E. coli. The OxyR transcription factor regulates the H₂O₂ response of E. coli. H₂O₂ oxidizes OxyR Cys199 to an R-SOH that reacts with Cys208 in an intramolecular disulphide bond.

Structure of the OxyR Regulatory Domain
(A)Reduced Form: Inactive
(B)Oxidized Form: Activated by ROS

_{Reference: D'Autréaux, B., & Toledano, M. B. (October 01, 2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8, 10, 813-824.}

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 H<sub>2</sub>O<sub>2</sub> to activate transcription, and is activated through the formation of an intramolecular disulfide bond.
-There are promoters that can be activated by OxyR: TrxCp([https://parts.igem.org/Part:BBa_K1104201 Part:BBa_K1104201]), HemHp([https://parts.igem.org/Part:BBa_K1104202 Part:BBa_K1104202]), ahpC([https://parts.igem.org/Part:BBa_K362005 Part:BBa_K362005])
+There are promoters that can be activated by OxyR: TrxCp([https://parts.igem.org/Part:BBa_K1104201 Part:BBa_K1104201]), HemHp([https://parts.igem.org/Part:BBa_K1104202 Part:BBa_K1104202]), ahpC, sufA([https://parts.igem.org/Part:BBa_K362005 Part:BBa_K362005])
 ===Literature Study of OxyR===