Difference between revisions of "Part:BBa K1132023:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1132023 short</partinfo>
 
<partinfo>BBa_K1132023 short</partinfo>
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===Design Notes===
 
===Design Notes===
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later
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A strong promoter with strong rbs was assembled to a preexisting biobrick rbs-TetR-term ([https://parts.igem.org/Part:BBa_P0440 BBa_P0440]) and permits to express TetR in great quantity. The presence of two ribosome binding sites before TetR coding sequence doesn't show any issue to express TetR. The system of TetR-pTet was described there : [https://parts.igem.org/Part:BBa_K1132022 BBa_K1132022]
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===Source===
 
===Source===
  
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later
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BBa_K608002<br> BBa_P0440
  
 
===References===
 
===References===

Latest revision as of 12:25, 1 October 2013

strong prom-strong rbs-rbs-tetR-term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A strong promoter with strong rbs was assembled to a preexisting biobrick rbs-TetR-term (BBa_P0440) and permits to express TetR in great quantity. The presence of two ribosome binding sites before TetR coding sequence doesn't show any issue to express TetR. The system of TetR-pTet was described there : BBa_K1132022


Source

BBa_K608002
BBa_P0440

References