Difference between revisions of "Part:BBa K1132023"
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| − | A strong promoter with strong rbs was assembled to a preexisting biobrick rbs-TetR-term (BBa_P0440) and permits to express TetR in great quantity. The presence of two ribosome binding sites before TetR coding sequence doesn't show any issue to express TetR. The system of TetR-pTet was described there : | + | A strong promoter with strong rbs was assembled to a preexisting biobrick rbs-TetR-term (BBa_P0440) and permits to express TetR in great quantity. The presence of two ribosome binding sites before TetR coding sequence doesn't show any issue to express TetR. The system of TetR-pTet was described there : [https://parts.igem.org/Part:BBa_K1132022 BBa_K1132022] |
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Revision as of 12:24, 1 October 2013
strong prom-strong rbs-rbs-tetR-term
A strong promoter with strong rbs was assembled to a preexisting biobrick rbs-TetR-term (BBa_P0440) and permits to express TetR in great quantity. The presence of two ribosome binding sites before TetR coding sequence doesn't show any issue to express TetR. The system of TetR-pTet was described there : BBa_K1132022
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]