Difference between revisions of "Part:BBa K1182425"
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This is an alpha fragment of beta galactosidase split enzyme. The enzyme beta galactosidase is expressed separately into two fragment, the alpha and omega fragment. When the alpha and omega fragment meets they will exhibit an enzymatic activity similar to the full length expressed beta galactosidase enzyme. | This is an alpha fragment of beta galactosidase split enzyme. The enzyme beta galactosidase is expressed separately into two fragment, the alpha and omega fragment. When the alpha and omega fragment meets they will exhibit an enzymatic activity similar to the full length expressed beta galactosidase enzyme. | ||
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| + | This split reporter can be used as biosensor using ternary complexation mediated protein complementation principle where the interaction of two proteins (in this case the alpha and omega fragment) is mediated by another protein. | ||
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Revision as of 19:27, 30 September 2013
Split β-galactosidase - α fragment
This is an alpha fragment of beta galactosidase split enzyme. The enzyme beta galactosidase is expressed separately into two fragment, the alpha and omega fragment. When the alpha and omega fragment meets they will exhibit an enzymatic activity similar to the full length expressed beta galactosidase enzyme.
This split reporter can be used as biosensor using ternary complexation mediated protein complementation principle where the interaction of two proteins (in this case the alpha and omega fragment) is mediated by another protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]