Difference between revisions of "Part:BBa K1060013"

Revision as of 09:49, 30 September 2013

LacI-AroG* production under regulation of TetR repressible promoter

Regulatory construct to get expression of LacI (BBa_C0012) and mutated AroG (for details see the wild-type AroG; BBa_K1060000) under the control of the TetR repressible promoter (BBa_R0040). The mutated AroG (Pro150Leu and Leu175Asp) is less repressed by phenylalanine resulting in a upscaling of the shikimate pathway and production of more chorismic acid (ChA). ChA being a precursor of methyl salicylate. A biosensor which expresses TetR (C0040) under the certain conditions could switch off LacI and mutated AroG expression.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1228
Illegal XhoI site found at 2195
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1502

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1060013 short</partinfo>
-Regulatory construct to get expression of LacI (<partinfo>BBa_C0012</partinfo>) and mutated AroG (for details see the wild-type AroG; <partinfo>Bba_K1060000</partinfo>) under the control of the TetR repressible promoter (<partinfo>Bba_R0040</partinfo>). The mutated AroG (Pro150Leu and Leu175Asp) is less repressed by phenylalanine resulting in a upscaling of the shikimate pathway and production of more chorismic acid (ChA). ChA being a precursor of methyl salicylate. A biosensor which expresses TetR (C0040) under the certain conditions could switch of LacI and mutated AroG expression.
+Regulatory construct to get expression of LacI (<partinfo>BBa_C0012</partinfo>) and mutated AroG (for details see the wild-type AroG; <partinfo>Bba_K1060000</partinfo>) under the control of the TetR repressible promoter (<partinfo>Bba_R0040</partinfo>). The mutated AroG (Pro150Leu and Leu175Asp) is less repressed by phenylalanine resulting in a upscaling of the shikimate pathway and production of more chorismic acid (ChA). ChA being a precursor of methyl salicylate. A biosensor which expresses TetR (C0040) under the certain conditions could switch off LacI and mutated AroG expression.
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