Difference between revisions of "Part:BBa K1111004:Experience"

(→‎Applications of BBa_K1111004)
(→‎Applications of BBa_K1111004)
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We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.
 
We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.
 
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Score Expect Identities Gaps Strand
 
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893 bits(483) 0.0 483/483(100%) 0/483(0%) Plus/Plus
 
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Query  1    CCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGA  60 <BR>
 
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Sbjct  72  CCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGA  131 <BR>
 
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Query  61  ATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCT  120
 
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Sbjct  132  ATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCT  191
 
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Query  121  GAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCAC  180
 
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Sbjct  192  GAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCAC  251
 
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Query  181  CATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCA  240
 
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            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 
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Sbjct  252  CATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCA  311
 
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Query  241  AAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTT  300
 
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Sbjct  312  AAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTT  371
 
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Query  301  GTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGA  360
 
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            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 
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Sbjct  372  GTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGA  431
 
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Query  361  AGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGAC  420
 
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            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 
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Sbjct  432  AGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGAC  491
 
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Query  421  GTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGGAGAGACGTGC  480
 
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            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 
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Sbjct  492  GTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGGAGAGACGTGC  551
 
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Query  481  GAT  483
 
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            |||
 
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Sbjct  552  GAT  554
 
  
 
===User Reviews===
 
===User Reviews===

Revision as of 09:33, 30 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1111004

We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.

User Reviews

UNIQ9227af1b22ea09be-partinfo-00000000-QINU UNIQ9227af1b22ea09be-partinfo-00000001-QINU