Difference between revisions of "Part:BBa K1150036"
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| − | This device can be used in combination with dCas9. It contains a DNA sequence that transcripts a crRNA that binds complementary to VEGF1 target ( | + | This device can be used in combination with dCas9. It contains a DNA sequence that transcripts a crRNA that binds complementary to VEGF1 target (TTAAAAGTCGGCTGGTAGCGGGGAGGATCG). |
==Biology and Usage== | ==Biology and Usage== | ||
Revision as of 23:38, 29 September 2013
uniCAS RNAimer to target VEGF1
| RNAimer (VEGF1 target) | |
|---|---|
| Function | Targeting VEGF1 with Cas9 |
| Use in | Mammalians |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
This device can be used in combination with dCas9. It contains a DNA sequence that transcripts a crRNA that binds complementary to VEGF1 target (TTAAAAGTCGGCTGGTAGCGGGGAGGATCG).
Biology and Usage
Freiburg 2013 used this plasmid to test the efficiency of target VEGF1. Therefore this target was also cloned in front of a promoter, that on its part is located in front of a SEAP reporter gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 224
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 216
Literature