Difference between revisions of "Part:BBa K1127016"

Revision as of 20:25, 29 September 2013

Gold and QS ultra complex

The device is a tunable hybrid bistable switch. It receives two signals 1. gold (HAuCl4) and 2. population density, which modify magnitude of the output 'transactivation of the target gene'.

Gold is recognized by the part BBa_K1127008 [1] which in response produces AiiA [2]. AiiA can degrade the quorum sensing signal AHL; therefore, it reduces expression of the target gene. LuxR [3] is produced constitutively by the promoter BBa_J23100 [4]. It is activated by AHL and forms a positive feedback loop to promote expression of the AHL synthases LuxI [5] and transactivating RNA taR12 [6].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1747
Illegal NheI site found at 1770
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3565
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 701
Illegal AgeI site found at 880
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2808

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 __NOTOC__
 <partinfo>BBa_K1127016 short</partinfo>
-Details to be written...
+The device is a tunable hybrid bistable switch. It receives two signals 1. gold (HAuCl4) and 2. population density, which modify magnitude of the output 'transactivation of the target gene'.
+Gold is recognized by the part BBa_K1127008 [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1127008] which in response produces AiiA [https://parts.igem.org/Part:BBa_K516022]. AiiA can degrade the quorum sensing signal AHL; therefore, it reduces expression of the target gene.
+LuxR [https://parts.igem.org/Part:BBa_I0462] is produced constitutively by the promoter BBa_J23100 [https://parts.igem.org/Part:BBa_J23100]. It is activated by AHL and forms a positive feedback loop to promote expression of the AHL synthases LuxI [https://parts.igem.org/Part:BBa_K805016] and transactivating RNA taR12 [https://parts.igem.org/Part:BBa_J01008].
 <!-- Add more about the biology of this part here