Difference between revisions of "Part:BBa K1149050"
Margarita K (Talk | contribs) |
|||
Line 9: | Line 9: | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | |||
+ | In addition to this, we have shown that bacterial paintings are achievable with this amazing biobrick (yellow): | ||
+ | [[File:Originals_combined_small.jpg|left|600px]] | ||
<!-- --> | <!-- --> |
Revision as of 18:40, 29 September 2013
Bdh2 (intracellular)
This part expresses Bdh2 3HB dehydrogenase enzyme intracellularly. There is a GFP in operon with the enzyme which can be used to monitor expression levels. The promoter is arabinose inducible pBAD.
http://www.igem.org/wiki/images/f/f0/Reaction_bdh.jpg
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 181
Illegal AgeI site found at 1126 - 1000COMPATIBLE WITH RFC[1000]