Difference between revisions of "Part:BBa K1151038"

Revision as of 18:04, 29 September 2013

Double generator NikR-GFP, IPTG-nickel regulated

A simple construct composed of K1151006 + K1151011, used for the NikR activity study.

Fluorescence decay assay

In both graphs it's evident the difference between the 3 controls sample (LB, LB+Ni2+, LB+IPTG) compared to the Ni-IPTG incubated sample in the time-dependent fluorescence signal variations.

The expression of the repressor, NikR (BBa_K1151000), under the plac promoter control, has effects on the fluorescence level of GFP only in the culture incubated with nickel. The response of repression of GFP is found in both the constructs with NikR-responsive promoters and it is quite clear despite the long half-life of GFP.

The proof showed the correct operation of the biobricks, in particular the good functionality of the repression mechanism, nickel-dependent, operated by NikR on the two promoters, that composed a divergent intergenic region of Helicobacter pylori, pnikr e pexbB.

Measurements were made at two typical wavelenghts of GFP to have a complete and secure framework of the experiments.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1737

@@ Line 10: / Line 10: @@
 [[File:gfp3.jpg]]                             [[File:gfp4.jpg]]
+In both graphs it's evident the difference between the 3 controls sample (LB, LB+Ni2+, LB+IPTG) compared to the Ni-IPTG incubated sample in the time-dependent fluorescence signal variations.
+The expression of the repressor, '''NikR ([[BBa_K1151000]])''', under the plac promoter control, has effects on the  fluorescence level of GFP only in the culture incubated with nickel. The response of repression of GFP is found in both the constructs with NikR-responsive promoters and it is quite clear despite the long half-life of GFP.
+The proof showed the correct operation of the biobricks, in particular the good functionality of the repression mechanism, nickel-dependent, operated by NikR on the two promoters, that composed a divergent intergenic region of Helicobacter pylori, pnikr e pexbB.
+''Measurements were made at two typical wavelenghts of GFP to have a complete and secure framework of the experiments.''
 ===Sequence and Features===