Difference between revisions of "Part:BBa K1111002"

Revision as of 16:33, 29 September 2013

Hya promoter and Superfolded GFP

Introduction

The hya promoter is encoded in the genome of K-12 MG1655 E.Coli bacteria. Upon external acidification it is activated and induced transcribtion of hydrogenase isoenzyme 1. This enzyme can then catalyze reversible oxidation of hydrogen.

Figure 1: From http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10468. How the hya promoter is induced

As a proof of principle we inserted the hya Promoter in front of BioBrick BBa_I746916 which encodes superfolded GFP. Then the induction was tested by transforming cells with the construct, innoculating them in media with different pHs and finally measure their GFP expression with a plate reader.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 184
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 184
Illegal NheI site found at 499
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 184
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 184
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 184
Illegal AgeI site found at 26
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 543

@@ Line 7: / Line 7: @@
 [[Image: HYA.gif|thumb|600px|center|Figure 1: From http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10468. How the hya promoter is induced ]]
+As a proof of principle we inserted the hya Promoter in front of BioBrick BBa_I746916 which encodes superfolded GFP. Then the induction was tested by transforming cells with the construct, innoculating them in media with different pHs and finally measure their GFP expression with a plate reader.
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