Difference between revisions of "Part:BBa K1223003:Design"

 
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===Design Notes===
 
===Design Notes===
−
selection for cassette integration into E.coli BL21 genome
+
selection gene for that confers the resistance to Kanamycin/Neomycin antibiotics. part is a synthetic construct also used in the registry's pSB3K5 plasmid backbone.
  
 +
this part was used as a selection gene for the P.A.S.E. 1 and 2 cassettes.
  
 +
this part contains the promoter, CDS and stop codon.
  
 
===Source===
 
===Source===
  
−
yeast
+
part was extracted from the pKD4 plasmid and is similar to entry EU496093 on Genbank.
  
 
===References===
 
===References===
 +
Shetty, R.; Endy, D.; Knight, T. Journal of Biological Engineering 2008, 2, 5.

Latest revision as of 16:28, 29 September 2013


Kanamicin ressistance


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

selection gene for that confers the resistance to Kanamycin/Neomycin antibiotics. part is a synthetic construct also used in the registry's pSB3K5 plasmid backbone.

this part was used as a selection gene for the P.A.S.E. 1 and 2 cassettes.

this part contains the promoter, CDS and stop codon.

Source

part was extracted from the pKD4 plasmid and is similar to entry EU496093 on Genbank.

References

Shetty, R.; Endy, D.; Knight, T. Journal of Biological Engineering 2008, 2, 5.