Difference between revisions of "Part:BBa K1073003"
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<partinfo>BBa_K1073003 short</partinfo> | <partinfo>BBa_K1073003 short</partinfo> | ||
| − | This construct is derived from <partinfo>BBa_K091117</partinfo> and <partinfo>BBa_B0032</partinfo>. It combines the inducible promoter of | + | This construct is derived from <partinfo>BBa_K091117</partinfo> and <partinfo>BBa_B0032</partinfo>. It combines the inducible promoter of the las Quorum sensing system of ''Pseudomonas aeruginosa'' with a RBS. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The inducible promoter from ''Pseudomonas aeruginosa'' is activated by LasR complexed with the specific autoinducer N-3-oxododecanoyl-HSL | + | The inducible promoter from ''Pseudomonas aeruginosa'' is activated by LasR complexed with the specific autoinducer N-3-oxododecanoyl-HSL synthesized by LasI. The induction of the promoter |
in ''P. aeruginosa'' leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003]. | in ''P. aeruginosa'' leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003]. | ||
The device can be cloned in front of a desired gene of interest in order to induce its expression by N-3-oxododecanoyl-HSL. | The device can be cloned in front of a desired gene of interest in order to induce its expression by N-3-oxododecanoyl-HSL. | ||
Latest revision as of 15:42, 29 September 2013
Inducible promoter of the las quorum sensing system with RBS
This construct is derived from BBa_K091117 and BBa_B0032. It combines the inducible promoter of the las Quorum sensing system of Pseudomonas aeruginosa with a RBS.
Usage and Biology
The inducible promoter from Pseudomonas aeruginosa is activated by LasR complexed with the specific autoinducer N-3-oxododecanoyl-HSL synthesized by LasI. The induction of the promoter in P. aeruginosa leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003]. The device can be cloned in front of a desired gene of interest in order to induce its expression by N-3-oxododecanoyl-HSL. For further Information see BBa_R0079.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]