Difference between revisions of "Part:BBa K1071004"
Line 3: Line 3:
  
 
Promoter for nitrate reductase in ''Phaeodactylum tricornutum''. The protein nitrate reductase catalyses the reduction from nitrate to nitrite. Nitrate is important nitrogen source for plants, therefore is the NR promotor tightly regulated. Only if nitrate is the only nitrogen source, the promotor will be active.
 
Promoter for nitrate reductase in ''Phaeodactylum tricornutum''. The protein nitrate reductase catalyses the reduction from nitrate to nitrite. Nitrate is important nitrogen source for plants, therefore is the NR promotor tightly regulated. Only if nitrate is the only nitrogen source, the promotor will be active.
−
In the lab is the promotor used to express heterologous proteins. The expression can be regulated by the amount of ammonia in the medium.
+
In the lab is the promotor used to express heterologous proteinsin ''P. tricornutum''. The expression can be regulated by the amount of ammonia in the medium.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:05, 29 September 2013

P NR

Promoter for nitrate reductase in Phaeodactylum tricornutum. The protein nitrate reductase catalyses the reduction from nitrate to nitrite. Nitrate is important nitrogen source for plants, therefore is the NR promotor tightly regulated. Only if nitrate is the only nitrogen source, the promotor will be active. In the lab is the promotor used to express heterologous proteinsin P. tricornutum. The expression can be regulated by the amount of ammonia in the medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 374
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]