Difference between revisions of "Part:BBa K1072010"
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| − | We fuse <bbpart>BBa_K1072008</bbpart> with plasma membrane protein,Odr-10 and expressed in yeast successfully figure | + | We fuse <bbpart>BBa_K1072008</bbpart> with plasma membrane protein,Odr-10 and expressed in yeast successfully figure 2. |
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Revision as of 08:56, 29 September 2013
Odr-10(diacetyl)GPCR with optimized codon
Odr-10(diacetyl)GPCR encodes a diacetyl-sensing G-protein coupled receptor, originating from the C. elegans.
If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein. pathway is composed of Gpa1 (Gα), Ste4 (Gβ) and Ste18 (Gγ) subunits.The Gβγ particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will links a reproter, such as GFP, His3 or LacZ.
Figure 1. a diagram of Odr-10, 7-transmembrane receptors.
We fuse BBa_K1072008 with plasma membrane protein,Odr-10 and expressed in yeast successfully figure 2.
Figure 2. fluorescence was detected around plasma membrane
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]