Difference between revisions of "Part:BBa K1072010"
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| − | We fuse | + | We fuse <bbpart>BBa_K1072008<bbpart> with plasma membrane protein,Odr-10 and expressed in yeast successfully figure 1. |
Revision as of 08:55, 29 September 2013
Odr-10(diacetyl)GPCR with optimized codon
Odr-10(diacetyl)GPCR encodes a diacetyl-sensing G-protein coupled receptor, originating from the C. elegans.
If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein. pathway is composed of Gpa1 (Gα), Ste4 (Gβ) and Ste18 (Gγ) subunits.The Gβγ particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will links a reproter, such as GFP, His3 or LacZ.
Figure 1. a diagram of Odr-10, 7-transmembrane receptors.
We fuse BBa_K1072008